Transcription Regulation System Mediated by Mechanical Operation of a DNA Nanostructure

Communication Transcription Regulation System Mediated by Mechanical Operation of a DNA Nanostructure Masayuki Endo*†§, Ryoji Miyazaki‡, Tomoko Emura‡, Kumi Hidaka‡, and Hiroshi Sugiyama*†‡§ † Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501, Japan. ‡ Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan § CREST, Japan Science and Technology Corporation (JST), Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan J. Am. Chem. Soc., Article ASAP DOI: 10.1021/ja2074856 Publication Date (Web): January 26, 2012 Copyright © 2012 American Chemical Society endo@kuchem.kyoto-u.ac.jp; hs@kuchem.kyoto-u.ac.jp Abstract

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A transcription regulation system initiated by DNA nanostructure changes was designed and constructed. Using the toehold system, specific DNA strands induced the opening of the tubular structure. A transcription product from the purified tube-attached dsDNA template was observed by addition of DNA strands that were specific for opening the tubular structure. http://pubs.acs.org/doi/abs/10.1021/ja2074856